The M65 EpiDeath ELISA is the next generation of Peviva's biomarkers. It is intended for the quantitative detection of keratin 18 (K18) present in cell culture supernatants, human serum, human plasma or other body fluids after it has been released from dying cells into the extracellular compartment. The assays offers the opportunity to quantify total cell death (due to apoptosis and necrosis) of K18 positive (epithelial) cells.
The M65 EpiDeath ELISA, when used in combination with the M30 Apoptosense ELISA (which specifically measures apoptosis), allows to distinguish different forms of epithelial cell death as both assay are standardised against the identical reference (Kramer et al., 2004).
The M65 EpiDeath ELISA is a modified version of the M65 ELISA and is intended for the samples with high Keratin 18 plasma and serum levels (e.g. sepsis, GvHD and liver toxicity such as DILI or ALF).